Establishment of a New Epstein - Barr Virus - Immortalized Cell Line From Chronic
نویسندگان
چکیده
C HRONIC LYMPHOCYTIC LEUKEMIA (CLL) B cells are, in general, highly refractory to transformation by Epstein-Barr virus (EBV),’ and therefore, relatively few EBV-immortalized cell lines originating from CLL cells have been described.’4 In this paper we report the establishment of a novel EBV-transformed B cell line from the leukemic cells of a CLL patient that expressed surface u immunoglobulin. A distinctive feature of these leukemic cells, which makes this cell line unique, is that their surface 1gM reacted with a sheep RBC (SRBC) antigen. Specific binding of SRBC was maintained by the EBV-transformed cells, which indicated their leukemic origin. This was futher confirmed by karyotypic analysis showing that the immortalized cells carried trisomy of chromosome I 2, a cytogenetic abnormality typical of CLL of the B cell type.5
منابع مشابه
Establishment of a new Epstein-Barr virus-immortalized cell line from chronic lymphocytic leukemia with trisomy of chromosome 12 that produces monoclonal IgM against a sheep RBC antigen.
Leukemia cells from a patient with chronic lymphocytic leukemia (CLL) were found to bind sheep RBC (SRBC) through their monoclonal surface IgM. A lymphoblastoid cell line was obtained by immortalization of leukemic cells with Epstein-Barr virus (EBV). Cultured leukemic cells were found to have a supernumerary chromosome 12, an abnormality typical of CLL of the B cell type. To our knowledge, thi...
متن کاملEstablishment of a New Epstein - Barr Virus - Immortalized Cell Line From Chronic Lymphocytic Leukemia With Trisomy of Chromosome 12
C HRONIC LYMPHOCYTIC LEUKEMIA (CLL) B cells are, in general, highly refractory to transformation by Epstein-Barr virus (EBV),’ and therefore, relatively few EBV-immortalized cell lines originating from CLL cells have been described.’4 In this paper we report the establishment of a novel EBV-transformed B cell line from the leukemic cells of a CLL patient that expressed surface u immunoglobulin....
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